Available Tian Lab AAV Expression Constructs
kLight (Opioid Receptor Based)
pAAV-hsyn-kLight0
Synapsin-driven κLight0, control sensor for kLight
pAAV-hsyn-kLight1.3
Synapsin-driven kappa opioid receptor sensor κLight1.3
pAAV-CAG-DIO-kLight1.3a
Cre-inducible κLight1.3a with MTRIA linkers with increased basal fluorescence
deltaLight (Opioid Receptor Based)
pAAV-hSyn-deltaLight
Synapsin-driven delta opioid receptor sensor δLight
pAAV-hSyn-deltaLight0
Synapsin-driven δLight0, the deltaLight control sensor
dLight (Dopamine Sensor)
pAAV-CAG-dLight3.6
High-affinity dopamine sensor dLight3.6 from the dLight3.0 series with CAG promoter
pAAV-CAG-DIO-dLight3.6
Cre-inducible high-affinity dopamine sensor dLight3.6 with CAG promoter
pAAV-ihSyn-tTA-sv40/TRE-dLight3.6
Sparse, but strong expression of high-affinity dopamine sensor dLight3.6. Synapsin promoter drives tTA expression to activate TRE-driven dLight3.6 expression.
pAAV-Syn-dLight3.8
High dynamic range dopamine sensor dLight3.8 from the dLight3.0 series with neuron-specific Synapsin promoter
pAAV-CAG-dLight3.8
High dynamic range dopamine sensor dLight3.8 from the dLight3.0 series with CAG promoter
pAAV-CAG-DIO-dLight3.8
Cre inducible high dynamic range dopamine sensor dLight3.8 with CAG promoter
pAAV-ihSyn-tTA-sv40/TRE-dLight3.8
Sparse but strong expression of high dynamic range dopamine sensor dLight3.8. Synapsin promoter drives tTA expression to activate TRE-driven dLight3.8 expression.
iSeroSnFR (Serotonin Sensors)
pAAV-hSyn-iSeroSnFR2.0GPI
Synapsin-driven high-affinity, high dynamic range version of iSeroSnFR with GPI membrane targeting.
pAAV-hSyn-iSeroSnFR0.0GPI
Synapsin-driven control construct with GPI transmembrane domain for iSeroSnFR that does not respond to serotonin.
pAAV-hSyn-iSeroSnFR1.4NLG
Synapsin-driven high-affinity version of iSeroSnFR with Neuroligin membrane targeting.
pAAV-hSyn-iSeroSnFR0.0Nlg
Synapsin-driven control construct with Neuroligin transmembrane domain for iSeroSnFR that does not respond to serotonin.