Available Tian Lab AAV Expression Constructs
kLight (Opioid Receptor Based)
pAAV-hsyn-kLight0
Synapsin-driven κLight0, control sensor for kLight
pAAV-hsyn-kLight1.3
Synapsin-driven kappa opioid receptor sensor κLight1.3
pAAV-CAG-DIO-kLight1.3a
Cre-inducible κLight1.3a with MTRIA linkers with increased basal fluorescence
deltaLight (Opioid Receptor Based)
pAAV-hSyn-deltaLight
Synapsin-driven delta opioid receptor sensor δLight
pAAV-hSyn-deltaLight0
Synapsin-driven δLight0, the deltaLight control sensor
dLight (Dopamine Sensor)
pAAV-CAG-dLight3.6
High-affinity dopamine sensor dLight3.6 from the dLight3.0 series with CAG promoter
pAAV-CAG-DIO-dLight3.6
Cre-inducible high-affinity dopamine sensor dLight3.6 with CAG promoter
pAAV-ihSyn-tTA-sv40/TRE-dLight3.6
Sparse, but strong expression of high-affinity dopamine sensor dLight3.6. Synapsin promoter drives tTA expression to activate TRE-driven dLight3.6 expression.
pAAV-Syn-dLight3.8
High dynamic range dopamine sensor dLight3.8 from the dLight3.0 series with neuron-specific Synapsin promoter
pAAV-CAG-dLight3.8
High dynamic range dopamine sensor dLight3.8 from the dLight3.0 series with CAG promoter
pAAV-CAG-DIO-dLight3.8
Cre inducible high dynamic range dopamine sensor dLight3.8 with CAG promoter
pAAV-ihSyn-tTA-sv40/TRE-dLight3.8
Sparse but strong expression of high dynamic range dopamine sensor dLight3.8. Synapsin promoter drives tTA expression to activate TRE-driven dLight3.8 expression.
iSeroSnFR (Serotonin Sensors)
pAAV-hSyn-iSeroSnFR2.0GPI
Synapsin-driven high-affinity, high dynamic range version of iSeroSnFR with GPI membrane targeting.
pAAV-hSyn-iSeroSnFR0.0GPI
Synapsin-driven control construct with GPI transmembrane domain for iSeroSnFR that does not respond to serotonin.
pAAV-hSyn-iSeroSnFR1.4NLG
Synapsin-driven high-affinity version of iSeroSnFR with Neuroligin membrane targeting.
pAAV-hSyn-iSeroSnFR0.0Nlg
Synapsin-driven control construct with Neuroligin transmembrane domain for iSeroSnFR that does not respond to serotonin.
AAV Stock Preps
(Other capsids available by custom order) available in 5ul aliquots
pAAV-hSyn-kLight1.3 AAV9
AAV-hSyn-deltaLight AAV9
pAAV-Syn-dLight3.8 AAV1
CAG-DIO-dLIGHT3.0A7 AAV1
CAG-DIO-dLight3.0A5 AAV1
CAG-DIO-dLight3.0A8-WPRE.sv40 AAV1
CAG-DIO-dLight3.0A6b-WPRE.sv40 AAV1
pAAV-CAG-dLight3.6 AAV1
pAAV-CAG-DIO-dLight3.6 AAV1
pAAV-CAG-DIO-dLight3.8 AAV1
hSyn-kLight1.3 AAV1
pAAV hSyn iSeroSnFR1.4NLG AAV1
pAAV hSyn iSeroSnFR 0.0 Nlg(mutant) AAV1
pAAV hSyn iSeroSnFR 0.0 GPI(mutant) AAV1
AAV-hSyn-deltaLight AAV1
pAAV-CAG-DIO-κLight1.3a AAV1
pAAV-hSyn-kLight0(mutant) AAV1
pAAV hSyn iSeroSnFR 2.0 GPI AAV1
AAV-hSyn-deltaLight0(mutant) AAV1
CAG-DIO-dLight3.0A8-WPRE.sv40 AAV1
iHSyn-ttA-tRE-hSyn-ntsLight2.0 AAV1
kLight (Opioid Receptor Based)
AAV1-hsyn-kLight0
AAV1-hsyn-kLight1.3
AAV1-CAG-DIO-kLight1.3a
In process.
deltaLight (Opioid Receptor Based)
AAV1-hSyn-deltaLight
AAV1-hSyn-deltaLight0
dLight (Dopamine Sensor)
AAV1-CAG-dLight3.6
Lot 240123-08 = 1E12 GC/ml
AAV1-CAG-DIO-dLight3.6
Lot 221021-8 = 3.63E12 GC/ml, Lot 240111-01 = 9.9E12 GC/ml
AAV1-ihSyn-tTA-sv40/TRE-dLight3.6
In process.
AAV1-Syn-dLight3.8
Lot 240123-01 = 1.2E12 GC/ml
AAV1-CAG-dLight3.8
pAAV-CAG-DIO-dLight3.8
Lot 221021-3 = 8.15E12 GC/ml, Lot 240208-02 = 6.3E12 GC/ml
AAV1-ihSyn-tTA-sv40/TRE-dLight3.8
In process.
iSeroSnFR (Serotonin Sensors)
AAV1-hSyn-iSeroSnFR2.0GPI
In process.
AAV1-hSyn-iSeroSnFR0.0GPI
In process.
AAV1-hSyn-iSeroSnFR1.4NLG
pAAV1-hSyn-iSeroSnFR0.0Nlg
In process.